Procedures to carry out Oxidase test
o
Soak a piece of filter paper in the reagent solution.
o
Scrape some fresh growth from the plate with a disposable loop or stick and rub onto the
filter paper or touch a colony with edge of paper.
o
Examine for blue colour within 10 seconds.
o
Add 2 drops of reagent to suspect colonies on an agar plate. Do not flood the plate.
o
Examine for blue colour within 10 seconds.
o
Dip swab into reagent and then touch colony.
o
Examine for blue colour within 10 seconds.
- Impregnated Oxidase Test Strip Method
o
Scrape some fresh growth from the plate with a disposable loop or stick and rub onto
o
the filter paper.
o
Examine for blue colour within 10 seconds.
Positive result development
of a blue colour indicates oxidase production
Negative result no blue
colour
