Make your own free website on Tripod.com

Biochemical Test

Urease test

Home
Biochemical Test
Agar Plates
Coagulase Test
The Catalase Test
Indole Test
Oxidase Test
Citrate Test
Optichin Test
Urease test
References

Urease Test

Urease is an enzyme that breaks the carbon-nitrogen bond of amides to form carbon dioxide, ammonia, and water. Members of genus Proteus are known to produce urease. Urease can be detected by plating bacteria onto an amide containing medium, specifically urea. When urea is broken down, ammonia is released and the pH of the medium increases. This pH change is detected by a pH indicator that turns pink in a basic environment. A pink medium indicates a positive test for urease.

ureasetest.jpg

Urease test

 

o    Inoculate slope heavily over the entire surface.

o    Incubate inoculated slope at 35-37C in a water bath or hot block or incubator.

o    Examine slopes after 4h and after overnight incubation.

 

Positive result purple/pink colour

Negative result colour of medium remains unchanged